書誌事項
- タイトル別名
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- Isolation and characterization of C-reactive protein and serum amyloid P component from bovine serum.
- 牛血清C-反応性タンパク質および血清アミロイドPタンパク質の分離および生化学的諸性状〔英文〕
- ウシ ケッセイ C ハンノウセイ タンパクシツ オヨビ ケッセイ アミロイド
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説明
C-reactive protein (CRP) and serum amyloid P component (SAP), which are known as acute phase reactants in human and many other animals, were purified from cow sera. Affinity chromatography using HE agarose gel was the most effective method to isolate both CRP and SAP from a large volume of bovine serum. Separation of CRP and SAP from the mixed preparation could be performed by DEAE-cellulose column chromatography, gel permeation HPLC using TSK-G3000SW or affinity chromatography using phosphorylcholine derivatives of bovine serum albumin-conjugated Toyopearl HW 65. Bovine CRP and SAP were identified as genuine CRP- and SAP-class proteins by their cross reactivities with anti-human CRP and anti-human SAP, respectively, and by their homology in amino acid compositions compared with those of human CRP and SAP, respectively. Bovine CRP moved slower than β-globulin, and bovine SAP moved in the β-globulin region in agarose gel electrophoresis. Both of them gave single bands in native polyacrylamide gel electrophoresis (PAGE). Bovine CRP and SAP molecular weights were estimated to be 100, 600 and 109, 500 daltons respectively, by sedimentation equilibrium analysis. Bovine CRP showed 23K dalton subunits by sodium laurylsulfate-PAGE and bovine SAP showed 28K and 32K dalton subunits, both of which were glycosylated and had identical amino acid compositions, indicating that both CRP and SAP molecules are pentamers. In fact, they appeared to have pentameric disk-like configurations in electronmicroscopical examination.
収録刊行物
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- 日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
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日本獸醫學雜誌(The Japanese Journal of Veterinary Science) 51 (4), 723-732, 1989
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681399023232
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- NII論文ID
- 110003918042
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- NII書誌ID
- AN00191788
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- COI
- 1:CAS:528:DyaK3cXivVOj
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- ISSN
- 18811442
- 00215295
- http://id.crossref.org/issn/00215295
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- NDL書誌ID
- 3249502
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- PubMed
- 2585927
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可