Application of glucose consumption test for evaluating blastogenesis in bovine lymphocytes.

DOI JASI Web Site Web Site PubMed 1 Citations

Bibliographic Information

Other Title
  • グルコース消費試験を応用したウシリンパ球の幼若化反応の測定について
  • グルコース消費試験を応用したウシリンパ球の幼若化反応の測定について〔英文〕
  • グルコース ショウヒ シケン オ オウヨウシタ ウシ リンパキュウ ノ ヨウジ

Search this article

Abstract

The transformation of mitogen stimulated bovine lymphocytes was studied using the glucose consumption test. After four days of incubation of lymphocytes, the glucose concentration in the culture medium was determined. The differences of glucose concentration between control and mitogen stimulated cultures indicated the blastogenetic activity of the lymphocytes. Of four mitogens used, phytohaemagglutinin and concanavalin A responded well whereas responces with lipopolysaccharide and pokeweed mitogen were weak. This simple method was useful in evaluating the blastogenesis of bovine lymphocytes for routine use.

Journal

Citations (1)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top