ボツリヌス菌毒素に対するモノクローナル抗体を用いるELISAの評価

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  • Evaluation of ELISA techniques for titration of monoclonal antibodies against botulinum toxin.
  • ボツリヌス菌毒素に対するモノクローナル抗体を用いるELISAの評価〔英文〕
  • ボツリヌスキン ドクソ ニ タイスル モノクローナル コウタイ オ モチイル

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To evaluate the systems of enzyme-linked immunosorbent assay (ELISA) for titration of monoclonal antibodies, a monoclonal antibody, E13, against Clostridiurn botulinum type E derivative toxin was examined for the binding property. E13 neutralizing the toxin did not bind the toxin but bound the toxoid fixed on the surface of the wells of the polystyrene assay plate. Preincubation of peroxidase-labeled E13 in a toxin solution suppressed the binding of E13 to the fixed toxoid. Radioimmunoassay demonstrated the direct binding of E13 to the toxin. When the surface of the wells was coated with polyclonal antibody against the toxin and the antigens were fixed on solid phase (sandwich ELISA), E13 reacted to both toxin and toxoid. These results indicate that the site of the toxin molecule binding E13 is masked by fixing onto the surface of polystyrene material, absorption of the toxin and toxoid to the surface may be attained in different modes, and random binding may result from formalinization of the toxin. Moreover, these findings may warn that the conventional ELISA in which the solid phase is coated directly with the antigen sometimes fails in finding the positive antibody reaction.

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