肥育乳用雄子牛及び肥育豚直腸糞便からのサルモネラの分離とそれらのプラスミドの安定性

DOI 日本農学文献記事索引 Web Site Web Site PubMed 被引用文献2件

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  • Isolation of salmonellae from apparently healthy fattening male dairy calves and fattening pigs and stability of plasmids in the isolates.
  • 肥育乳用雄子牛及び肥育豚直腸糞便からのサルモネラの分離とそれらのプラスミドの安定性〔英文〕
  • ヒイク ニュウヨウ ユウ コウシ オヨビ ヒイク ブタ チョクチョウ フンベン

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Rectal contents from apparently healthy fattening male dairy calves and fattening pigs in a large geographical area in Japan were examined for the isolation of salmonellae between Aug. and Sept. 1982. Seven (2.46%) of 285 calves and 3 (1.05%) of 285 pigs were proved to be positive for salmonellae with 3 serovars. Six strains of Salmonella serovar typhimurium and 1 strain of S. serovar livingstone from calves, and 1 strain of S. serovar typhimurium and 2 strains of S. serovar derby from pigs were isolated. Except for 1 strain of S. serovar livingstone, all strains harbored at least 2 plasmids. By agarose gel electrophoresis, plasmid patterns of 5 strains of S. serovar typhimurium from calves and 2 strains of S. serovar derby from pigs were identical, respectively, although they were isolated in geographically independent sources. These plasmids, except for Inc Hl R plasmid detected in 1 strain of S. serovar typhimurium from a calf, were stable by the treatment with three-days passage at 43°C, ten-days passage at 37°C, ethidium bromide (1 mg/ml), sodium dodecyl sulfate (10%) and novobiocin (400 μg/ml).

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