Basic Study on Development of Artificial Membrane Vaccine Using Inter-membrane Protein Transfer.

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  • Yokota Satoshi
    Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
  • Tamaki Shinya
    Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
  • Hayashi Kyoko
    Faculty of Medicine, Toyama Medical and Pharmaceutical University
  • Ueno Masaharu
    Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

Bibliographic Information

Other Title
  • 膜タンパク質の膜間移行現象を利用した人工膜ワクチン調製法の検討
  • マク タンパクシツ ノ マク カン イコウ ゲンショウ オ リヨウ シタ ジンコウ マク ワクチン チョウセイホウ ノ ケントウ

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Abstract

Membrane protein transfer from influenza virus-infected cells to liposomes was examined in several experimental conditions using gel-filtration, SDS PAGE and fluorescent microscopy. In gel-filtration it was found that membrane protein spontaneously transferred from cell membrane to liposomal membrane during incubation of liposomes with cells, accompanied by some leaks of non-membrane protein.The amount of membrane protein incorporated into liposomal membrane was proportional to that of total protein released from cells. SDS PAGE experiment after immunoprecipitation confirmed the transfer of influenza antigenic protein from influenza virus-infected cell's membrane to liposomal membrane. Fluorescent image was observed all around liposome surface in microscopy using fluorescence antibody, suggesting the transferred antigenic protein was incorporated throughout into liposomal membrane.

Journal

  • MEMBRANE

    MEMBRANE 27 (2), 94-100, 2002

    THE MEMBRANE SOCIETY OF JAPAN

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