Effects and Neuro-toxic Mechanisms of 2,2',4,4',5,5'-Hexachlorobiphenyl and Endosulfan in Neuronal Stem Cells.
-
- KANG Kyung-Sun
- Department of Veterinary Public Health, School of Agricultural Biotechnology, College of Veterinary Medicine, Seoul National University
-
- PARK Jie-Eun
- Department of Veterinary Public Health, School of Agricultural Biotechnology, College of Veterinary Medicine, Seoul National University
-
- RYU Doug-Young
- Department of Environmental Science, School of Agricultural Biotechnology, College of Veterinary Medicine, Seoul National University
-
- LEE Yong-Soon
- Department of Veterinary Public Health, School of Agricultural Biotechnology, College of Veterinary Medicine, Seoul National University
この論文をさがす
抄録
Endocrine disrupters are exogenous compounds thought to mimic the action of estrogen or other hormones and influence endocrine activity in the body (Juberg, 2000). These chemicals have adverse effects not only in the reproductive system but also in the central nervous system during development and throughout life. Polychlorinated biphenyls (PCBs) are a class of environmentally persistent and widespread halogenated hydrocarbons. It has been reported that PCBs are potential neurotoxicants. Endosulfan is an organochlorine insecticide that is extensively used to control pests in vegetables, cotton, and fruits. To determine the effect of 2, 2', 4, 4', 5, 5',-hexachlorobiphenyl(2, 4, 5-HCB) and endosulfan on embryo nervous system, we isolated neural stem cells from rat brain at embryonic day 17. Isolated neural stem cells showed pluripotenty. Stem cells could differentiate into neurons and glia. Neurite formation in endosulfan and 2, 4, 5-HCB treated cells. And it appeared to be decreased as compared with that in untreated cells. In order to know the neuro-toxic mechanisms of 2, 4, 5-HCB and endosulfan in neuronal stem cells, we investigated mitogen-activated protein kinase activity (MAPK) and gap junctional intercellular communication (GJIC). Endosulfan decreased the MAPK activity in dose dependent manner. Endosulfan and 2, 4, 5-HCB inhibited GJIC compared to the untreated cell by scrape loading dye transfer (SL/DT). 2, 4, 5-HCB and endosulfan decreased the expression of connexin 43 in dose dependent manner. These results indicated that 2, 4, 5-HCB and endosulfan may inhibit differentiation and proliferation of neural stem cells and gap junctional intercellular communication which play a crucial role in the maintenance of cellular homeostasis.
収録刊行物
-
- The Journal of Veterinary Medical Science
-
The Journal of Veterinary Medical Science 63 (11), 1183-1190, 2001
公益社団法人 日本獣医学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681400829184
-
- NII論文ID
- 110003920772
-
- NII書誌ID
- AA10796138
-
- COI
- 1:CAS:528:DC%2BD38Xhs12jug%3D%3D
-
- ISSN
- 13477439
- 09167250
-
- NDL書誌ID
- 5984381
-
- PubMed
- 11767051
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可