Genetic Diversity of the Genes Encoding the Outer Membrane Lipoprotein(omlA) of Actinobacillus pleuropneumoniae.
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- OSAKI Makoto
- National Institute of Animal Health
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- SATO Yuko
- Kenhoku Livestock Hygiene Service Center
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- TOMURA Hidenori
- Ohmiya Livestock Hygiene Service Center
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- ITO Hiroya
- National Institute of Animal Health
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- SEKIZAKI Tsutomu
- National Institute of Animal Health
書誌事項
- タイトル別名
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- Genetic diversity of the genes encoding
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抄録
An allelic variation of the genes encoding the protective outer membrane lipoprotein (omlA) in Actinobacillus pleuropneumoniae was investigated with polymerase chain reaction (PCR)-restriction fragment length polymorphisms. Primers for PCR were selected from a conserved sequence compared between the omlA genes of A. pleuropneumoniae serotypes 1 and 5a. A DNA fragment of 970 bp was amplified from the genomic DNA of all 12 serotypes of A. pleuropneumoniae. The amplified DNA sequence specifically hybridized under a low stringent condition to the cloned omlA gene of A. pleuropneumoniae. Digestion of the amplified DNA with the enzymes either HinfI or VspI yielded specific polymorphic patterns, allowing discrimination of all serotypes into five distinct groups.
収録刊行物
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- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
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Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 59 (3), 213-215, 1997
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681401012608
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- NII論文ID
- 110003915127
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DyaK2sXisFGju7w%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 4173491
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- PubMed
- 9101482
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可