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- SHIMOJIMA Masayuki
- Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- NISHIMURA Yorihiro
- Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University
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- MIYAZAWA Takayuki
- Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University Host and Defense, PRESTO, Japan Science and Technology Corporation (JST)
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- KATO Kentaro
- Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- TOHYA Yukinobu
- Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- AKASHI Hiroomi
- Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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説明
The neural-cell adhesion molecule (N-CAM) consists of three major types of polypeptides (180, 140, and 120 kDa) whose predominant differences exist within the transmembrane and cytoplasmic domains. In this study, we generated a monoclonal antibody, termed SZK1, reactive to feline CD56 (fCD56) molecules (140 kDa form of N-CAM) expressed by the baculovirus expression system and investigated fCD56 expression in feline lymphoid cells. In flow cytometric analysis, SZK1 was reactive to a feline T-lymphoblastoid cell line MYA-1. Further, SZK1 was reactive to a very small population (1.1-1.7%) of freshly isolated peripheral blood lymphocytes (PBLs) of three specific pathogen-free cats, and the reactivity was increased by culturing of PBLs in the presence of interleukin-2 following concanavalin A-stimulation (>10%). In immunoblotting analysis, SZK1 detected an approximately 160 kDa antigen from MYA-1 cells, while from RNA of the cells reverse transcription-polymerase chain reaction amplified the fragment resembling 140 kDa form of N-CAM. These finding suggest that fCD56 has similar characteristics with human CD56.<br>
収録刊行物
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- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
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Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 65 (7), 769-773, 2003
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681401187584
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- NII論文ID
- 110003886255
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD3sXmvFWnurs%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 6653715
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- PubMed
- 12939502
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可
