METHODS FOR ESTIMATION OF ASPARTATE AND ALANINE-AMINOTRANSFERASE ACTIVITIES IN FOWL BLOOD PLASMA AND THEIR APPLICATION

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  • 鶏の血漿中 Aspartate-Alanine-aminotransferase 系アミノ基転移酵素の活性測定法について
  • ニワトリ ノ ケッショウ チュウ Aspartate- , Alanine-aminotransferaseケイ アミノキ テンイ コウソ ノ カッセイ ソクテイホウ ニ ツイテ

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Abstract

In man, the presence of aspartate-aminotransferase (GOT) and alanine-aminotrans-ferase (GPT), which are commonly known as the transaminases, is valued as a clue for the rapid diagnosis of myocarditis and hepatitis. The reversible transamination reactions by these transaminases, generally known in the replacement of =C=O with NH3, are essential for the utilization of nitrogen in living materials. So far as the author is aware, no detailed investigations have been conducted on the activities of GOT and GPT which are contained in the fowl blood plasma. For the purpose of studying this problem, the author tried to classify the abovementioned transaminases into categories bearing such terms as forward-GOT(F-GOT), reverse-GOT(R-GOT), forward-GPT (F-GPT), and reverse-GPT(R-GPT). Their activities were estimated by the atuhor's method, which was the same in principle as that reported by REITMAN and FRANKEL. The systems which are catalyzed by these transaminases are as follows.

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