Monoclonal Antibody TSd-1 is Specific to Elongating and Matured Spermatids in Testis of Common Tree Shrew(Tupaia glis).
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- MAEDA Seishi
- Department of Veterinary Anatomy, The University of Tokyo
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- NAKAMUTA Nobuaki
- Department of Veterinary Anatomy, The University of Tokyo
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- NAM Sang-Yoon
- Department of Veterinary Anatomy, The University of Tokyo
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- KIMURA Junpei
- Department of Veterinary Anatomy, Nihon University
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- ENDO Hideki
- Department of Zoology, National Science Museum
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- RERKAMNUAYCHOKE Worawut
- Department of Veterinary Anatomy, Kasetsart University
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- KUROHMARU Masamichi
- Department of Veterinary Anatomy, The University of Tokyo
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- YAMADA Junzo
- Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine
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- HAYASHI Yoshihiro
- Department of Veterinary Anatomy, The University of Tokyo
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- NISHIDA Takao
- Laboratory of Anatomy and Physiology, Nihon University
Bibliographic Information
- Other Title
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- Monoclonal antibody TSd-1 is specific t
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Abstract
The monoclonal antibody (MAb), named TSd-1, specific to spermatogenic cells of the common tree shrew (Tupaia glis) was established and characterized using immunohistochemistry and immunoblotting. MAb TSd-1 reacted with elongating and matured spermatids in a stage-dependent manner. TSd-1 recognized a 94 kilodalton (kDa) peptide in the plasma membrane and cytosol. Additionally, an extremely weak 107 kDa band was detected only in the cytosol. The reactions were not detected in round spermatids. In elongating Stage VI spermatids, the plasma membrane and the granular structure within the cytoplasm were intensely positive, and most intense after the appearance of new round spermatids in the lower layer (Stage I). The reactions were observed neither in the other organs of the common tree shrew nor in the testes of other animals, indicating that TSd-1 antigen is specific to the spermatogenic cells of the common tree shrew, and may act on elongating or matured spermatids.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 60 (3), 345-349, 1998
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681401206656
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- NII Article ID
- 110003918434
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DyaK1cXisVKisLg%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 4437743
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- PubMed
- 9560784
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed