Serum Progesterone Concentrations using P-EIA Kit in Captive and Free-Ranging Hokkaido Brown Bears, Ursus arctos yesoensis.
Bibliographic Information
- Other Title
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- 飼育下および野生エゾヒグマUrsus arctos yesoensisにおけるP-EIAキットによる血清中プロジェステロン値の測定
- 飼育下および野生エゾヒグマUrsus arctos yesoensisにおけるP-EIAキットによる血清中プロジェステロン値の測定〔英文〕
- シイクカ オヨビ ヤセイ エゾヒグマ Ursus arctos yesoens
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Abstract
Serum progesterone (P) concentrations using P-EIA kit (Ovucheck, Cambridge Life Science Co., Ltd.) were examined in 8 captive and 7 free-ranging female Hokkaido brown bears (Ursus arctos yesoensis). The intra- and inter-assay coefficients of variation were 8.9%, 12.6% and 16.6%, 22.7%, respectively, bdsed on 2 serum samples. There was a significant correlation between EIA and radioimmunoassay results based on 64 serum samples (r=0.725; p<0.01). Serum P concentrations were examined in 5 pregnant, 2 solitary non-pregnant bears and a lactating non-pregnant bear in captivity. Annual changes of P levels in pregnant bears were observed as a small elevation during the mating season (May-June), a re-elevation in September-October and a sharp elevation in November-December. The sharp elevation was suspected to reflect changes when implantation occurred. Annual changes of P levels in solitary non-pregnant bears were similar to those in pregnant bears. An annual change of P levels in a lactating non-pregnant bear maintained levels under 5 ng/ml. Two of 7 free-ranging bears exhibited P levels over 1 ng/ml and the birth of cubs was confirmed in the following year in 1 of the 2 bears. P concentrations of other free-ranging bears exhibited less than 1 ng/ml, and these bears were considered to be non-pregnant. It was concluded that P-EIA kit was available for measuring P concentrations in Hokkaido brown bears.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 54 (1), 1-5, 1992
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681401239296
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- NII Article ID
- 130003426162
- 110003915514
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DyaK38XksVSlsr4%3D
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- ISSN
- 13477439
- 09167250
- http://id.crossref.org/issn/09167250
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- NDL BIB ID
- 3761714
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- PubMed
- 1558871
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed