Identification of Endotrypanum Species from a Sloth, a Squirrel and Lutzomyia Sandflies in Ecuador by PCR Amplification and Sequencing of the Mini-Exon Gene
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- KATAKURA Ken
- Department of Parasitology, Gunma University School of Medicine
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- MIMORI Tatsuyuki
- Department of Tumor Genetics and Biology, Kumamoto University School of Medicine
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- FURUYA Masato
- Institute for Laboratory Animals
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- UEZATO Hiroshi
- Department of Dermatology, Faculty of Medicine, University of Ryukyus
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- NONAKA Shigeo
- Department of Dermatology, Faculty of Medicine, University of Ryukyus
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- OKAMOTO Munehiro
- Department of Laboratory Animal Science, School of Veterinary Medicine, Faculty of Agriculture, Torrori University
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- GOMEZ L. Eduardo A.
- Departamento de Medicina Tropical, Facultad de Medicina, Universidad Catolica Santiago de Guayaquil
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- HASHIGUCHI Yoshihisa
- Department of Parasitology, Kochi Medical School
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Abstract
PCR amplification and nucleotide sequencing of the mini-exon gene revealed that four strains isolated from a sloth (Choloepus hoffmanni), a squirrel (Sciurus granatensis) and two sandflies (Lutzomyia hartmanni) in Ecuador were indistinguishable from Endotrypanum monterogeii. Another strain isolated from Lu. hartmanni showed the high sequence similarity to E. schaudinni. Since three of these strains have been previously identified as Leishmania (Viannia) equatorensis, the results demonstrate that L. (V.) equatorensis is genetically closely related to the genus Endotrypanum. The present study also indicates that Endotrypanum species are distributed in arboreal animals and sandflies in Ecuador, and that mini-exon gene amplification is useful for epidemiological studies of Leishmania and Endotrypanum in the New World.<br>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 65 (5), 649-653, 2003
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681401536256
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- NII Article ID
- 130000448939
- 110003886231
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD3sXkvFKis74%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 6539874
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- PubMed
- 12808222
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed