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- KOMINE Yumiko
- Intelligent Cosmos Research Institute Corporation
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- KUROISHI Toshinobu
- Intelligent Cosmos Research Institute Corporation Division of Oral Immunology, Department of Oral Biology, Tohoku University Graduate School of Dentistry
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- KOBAYASHI Jin
- Department of Animal Reproduction, Miyagi University Research Farm
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- ASO Hisashi
- Cellular Biology Laboratory, Faculty of Agriculture, Tohoku University
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- OBARA Yoshiaki
- Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University
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- KUMAGAI Katsuo
- Department of Microbiology, Tohoku University Graduate School of Dentistry
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- SUGAWARA Shunji
- Division of Oral Immunology, Department of Oral Biology, Tohoku University Graduate School of Dentistry
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- KOMINE Ken-ichi
- Intelligent Cosmos Research Institute Corporation Division of Oral Immunology, Department of Oral Biology, Tohoku University Graduate School of Dentistry
書誌事項
- タイトル別名
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- Inflammatory Effect of Cleaved Bovine Lactoferrin by Elastase on Staphylococcal Mastitis
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抄録
Elastase activity and concanavalin A (Con A) low affinity bovine lactoferrin (bLf) molecule were detected in mammary gland secretions (MGSs) from mammary glands (MGs) with clinical staphylococcal mastitis. Changes in clinical symptoms correlated with increases in both elastase activity and the concentration of Con A low-affinity Lf in MGSs from mastitic MGs. Bovine Lf treated with elastase (elastase-Lf) showed various small bLf molecules and the same image on Con A two-dimensional immunoelectrophoresis as low Con A affinity bLf in MGSs. We confirmed the presence of four common bLf peptides for the elastase-bLf and low Con A affinity bLf molecules in mastitic MGSs, and synthesized four peptides. Strong mRNA expression of interleukin-6 (IL-6), tumor necrosis factor α (TNFα), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) was induced in bovine mammary epithelial cells on stimulation with low Con A affinity bLf, elastase-bLf, and GQRDLLFKDSAL, a synthesis bLf peptide based on nuclear factor kappa B (NFκB) activation. These results suggest that bLf was cleaved by elastase, and that this cleavage changed the physical function of Lf. Our results indicate that elastase induced production of low Con A affinity bLf, including the bLf peptide GQRDLLFKDSAL, and had an inflammatory effect on staphylococcal mastitis.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 68 (7), 715-723, 2006
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681401768960
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- NII論文ID
- 110004763181
- 130000447760
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 8044331
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可