ウマ卵巣インヒビンαサブユニットcDNAのクローニング

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タイトル別名
  • Molercular cloning of cDNA for inhibin .ALPHA.-subunit from equine ovary.
  • ウマ卵巣インヒビンαサブユニットcDNAのクローニング〔英文〕
  • ウマ ランソウ インヒビン アルファ サブ ユニット c DNA ノ クローニ

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抄録

cDNA encoding equine inhibin α-subunit precursor protein was isolated from an equine ovarian cDNA library. For screening, the DNA probe was amplified by the RT-PCR using primers designed based on the rat inhibin α-subunit cDNA sequence. Out of 1.2×105 plaques screened, 19 positive clones were isolated, and one of these clones (Eq-α-11) contained a complete open reading frame encoding 367 amino acids. The similarity of the deduced amino acid sequences of both equine inhibin a-subunit precursor protein and the mature protein were greater than 80% to those of other six mammalian species, including bovine, porcine, ovine, human, rat and mouse. Northern blot analysis revealed that a single major band at 1.5 kb was present in the RNA samples both from equine follicles and testes.

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