書誌事項
- タイトル別名
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- Identification of Skeletal Muscle Satellite Cells by Transfecting EGFP Driven by Skeletal .ALPHA.-Actin Promoter.
- Identification of skeletal muscle satellite cells by transfecting EGFP driven by skeletal α-actin promoter
- Identification of skeletal muscle satellite cells by transfecting EGFP driven by skeletal アルファ actin promoter
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In isolating skeletal muscle satellite cells, sometimes a problem is encountered in removing contaminating nonmyogenic cells.In the present study, we constructed a novel vector, pSKA−EGFP, which achieves the expression of enhanced green fluorescent protein(EGFP)exclusively in myogenic cells under the control of skeletal α−actin promoter when transfected to primary cultured cells from skeletal muscle.Cells from rat skeletal muscle positive for EGFP after transfecting with pSKA−EGFP were all positive for desmin and none of the nonmyogenic cells expressed EGFP, indicating that the expression of EGFP is specific to myogenic cells.Among the cells positive for EGFP were proliferating cells, presumably satellite cells.In addition, EGFP positive cells derived from horse skeletal muscle after transfecting pSKA−EGFP in vitro formed multinuclear myotubes, indicating that myogenic expression of EGFP driven by skeletal α−actin was achieved also in the equine cells.These results indicated that pSKA−EGFP vector will be useful in identifying and following up the satellite cells in real time, and also permit us to isolate satellite cells in combination with fluorescence−activated cell sorting(FACS).
収録刊行物
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- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
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Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 62 (11), 1213-1216, 2000
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681402203264
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- NII論文ID
- 110003920497
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD3cXovVGgt78%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 5577631
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- PubMed
- 11129869
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可