Determination of Nitrotyrosine by HPLC-ECD and Its Application.
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- ISHIDA Nobushige
- Equine Research Institute, Japan Racing Association
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- HASEGAWA Telhisa
- Equine Research Institute, Japan Racing Association
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- MUKAI Kazutaka
- Equine Research Institute, Japan Racing Association
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- WATANABE Masanobu
- Eicom Corporation
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- NISHINO Hirohito
- Eicom Corporation
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Description
3-nitrotyrosine, a product of tyrosine nitration, is a useful indicator of oxidative damage. We modified the previously reported HPLC-electrochemical detection (ECD) method: specifically, a through-type porous carbon electrode was used as a reducing electrode instead of the mercury-gold amalgam electrode, because the response of the latter changes over time. A combination of reverse-phase HPLC and electrochemical detector passed through -800 mV reduction potential and subsequently under +250 mV oxidation potential allows measurement of 3-nitrotyrosine. The detection limit of this assay was less than 10 fmol. In mice to which lipopolysaccharide (LPS) was administered intraperitoneally, plasma 3-nitrotyrosine levels were elevated, corresponding to LPS dosage. These findings suggest that the improved HPLC-ECD method can be used as a specific and sensitive assay of biological 3-nitrotyrosine and can be applied clinically.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 64 (5), 401-404, 2002
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681402230656
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- NII Article ID
- 110003920865
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD38Xlt1arsb4%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 6151581
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- PubMed
- 12069071
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed