Molecular Cloning of the Canine c-Met/HGF Receptor and Its Expression in Normal and Regenerated Liver

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  • NEO Sakurako
    Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University
  • KANSAKU Norio
    Laboratory of Animal Genetics and Breeding, School of Veterinary Medicine, Azabu University
  • FURUICHI Mitsuru
    Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University
  • WATANABE Masashi
    Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University
  • HISAMATSU Sin
    Department of Environmental Chemistry, College of Environmental Health, Azabu University
  • OHNO Koichi
    Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • HISASUE Masaharu
    Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University
  • TSUCHIYA Ryo
    Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University
  • YAMADA Takatsugu
    Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University

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  • 犬c-Met/HGFレセプターcDNAクローニングと組織分布ならびに再生肝におけるmRNAの発現

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The c-Met proto-oncogene is the receptor for hepatocyte growth factor (HGF), which is a member of the tyrosine kinase family. Activation of the HGF/c-Met signal pathway leads to cell proliferation, motility, regeneration, and morphogenesis. In this study, the complete nucreotide sequence of complementary DNA (cDNA) of canine c-Met was cloned, and its distribution was determined in tissues. The canine c-Met cDNA clone had an open reading frame of 4419 bp that encoded a putative polypeptide of 1383 amino acids. The c-Met mRNA was expressed in a variety of canine tissues including peripheral blood mononuclear cells (PBMC), bone marrow, liver, kidney, lung, stomach, uterus, testis, thymus, lymph node, small intestine, colon, adrenal gland, thyroid gland, heart, muscle, skin, pancreas, ovary, prostate, spleen, fat, cerebrum, and cerebellum. In addition, the c-Met mRNA expression in normal and regenerated liver was examined. The levels of the mRNA increased 2-fold in regenerated liver compared to that found in normal liver, indicating that c-Met is involved in various functions including remodeling of canine hepatocytes.<br>

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