Characterization of a Neutralizing Monoclonal Antibody against Botulinum ADP-Ribosyltransferase, C3 Exoenzyme.

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  • KAMATA Yoichi
    Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
  • HOSHI Hidenobu
    Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
  • CHOKI Hayato
    Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
  • KOZAKI Shunji
    Department of Veterinary Science, College of Agriculture, Osaka Prefecture University

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  • Bacteriology: Characterization of a neutralizing monoclonal antibody against botulinum ADP-ribosyltransferase, C3 exoenzyme

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A monoclonal antibody, named C302, was prepared and characterized against botulinum ADP-ribosyltransferase C3 exoenzyme that inactivates RhoA GTP-binding protein, resulting in the neurite outgrowth of human neuroblastoma GOTO cells. C302 bound not to the smaller fragments derived from the protease-treated C3 exoenzyme but to the intact C3 exoenzyme. It seems that the C302 epitope may depend on the three-dimensional structure of C3 exoenzyme molecule. C302 depressed the enzymatic and biological actions of C3 exoenzyme. The dose-dependent depression pattern of C302 on the enzyme activity was similar to that to the biological one. C302 turned the neurite-bearing shape of the C3 exoenzyme-treated GOTO cells into the intact shape. By using of C302 mAb and C3 exoenzyme, the research concerning GTP-binding proteins would be improved.

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