Lymphoid Apoptosis in Acute Canine Distemper
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- KUMAGAI Kazuyoshi
- Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University
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- YAMAGUCHI Ryoji
- Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University
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- UCHIDA Kazuyuki
- Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University
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- TATEYAMA Susumu
- Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University
Bibliographic Information
- Other Title
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- 犬ジステンパー急性感染期におけるリンパ組織のアポトーシス
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Description
The relationship between the canine distemper virus (CDV) infection and apoptosis in the canine lymphoid tissues was investigated using immunostaining for single stranded DNA (ssDNA), TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method, and electron microscopy. Twenty-six lymphoid tissues from 8 spontaneously CDV-infected dogs and 1 non-infected dog were used, and lesions were classified into 4 groups according to frequency of the CDV-antigen. Histologically, the degree of lymphoid depletion tended to depend on amount of CDV antigen. The numbers of ssDNA- and TUNEL-labeling cells were significantly high in the lymphoid tissues with abundant viral antigen. However, ssDNA- and TUNEL-positive lymphocytes were also frequently found even in the lymphoid tissues where there was only a small amount of CDV-antigen in sinus histiocytes. The incidence and distribution of apoptotic cells in the CDV-antigens-negative lymphoid tissues from infected dogs were equal to those from a non-infected dog. Double labeling immunostaining using a ssDNA and a CDV nucleocapsid protein (CDV-NP) antibody revealed that there were ssDNA positive but CDV-NP negative cells besides those stained doubly positive. Ultrastructurally, lymphocytes in the CDV-infected lymphoid tissues frequently had characteristic morphological features of apoptosis such as apoptotic bodies. All these results suggest that CDV leads to lymphocytic apoptosis directly or indirectly, resulting in severe lymphoid depletion and immunosuppression in acute or subacute phase of CDV infection.<br>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 66 (2), 175-181, 2004
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681403396480
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- NII Article ID
- 130000448712
- 110003886375
- 120007123344
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- NII Book ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- HANDLE
- 10458/1190
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- NDL BIB ID
- 6863852
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- PubMed
- 15031546
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- JaLC
- IRDB
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed