Inhibition of Rho-Associated Kinase Reduces MLC20 Phosphorylation and Contractility of Intact Myometrium and Attenuates Agonist-Induced Ca2+ Sensitization of Force of Permeabilized Rat Myometrium.
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- OH Jae-Hwan
- Department of Surgery, Gachon Medical School, Gil Medical Center
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- YOU Sung-Kyung
- Department of Physiology, College of Medicine, BK 21 Project for Medical Sciences, Yonsei University
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- HWANG Mi-Kyung
- Department of Physiology, College of Medicine, BK 21 Project for Medical Sciences, Yonsei University
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- AHN Duck-Sun
- Department of Physiology, College of Medicine, BK 21 Project for Medical Sciences, Yonsei University
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- KWON Seong-chun
- Department of Physiology, College of Medicine, BK 21 Project for Medical Sciences, Yonsei University
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- TAGGART Michael J.
- Department of Medicine, Manchester Royal Infirmary The Maternal & Fetal Health Research Centre, St Mary's Hospital, University of Manchester
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- LEE Young-Ho
- Department of Physiology, College of Medicine, BK 21 Project for Medical Sciences, Yonsei University
Bibliographic Information
- Other Title
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- Inhibition of rho-associated kinase reduces MLC20 phosphorylation and contractility of intact myometrium and attenuates agonist-induced Ca〔2+〕 sensitization of force of permeabilized rat myometrium
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Abstract
The role of rhoA/rho-associated kinase (ROK) signaling pathways in agonist-induced contraction of the rat myometrium was investigated. We measured the [Ca2+]i-force relationship, phosphorylation of myosin regulatory light chains (MLC20) in intact tissue and the Ca2+-sensitization of force in permeabilized myometrial cells of rat. In measurements of the relationship between [Ca2+]i and tension in intact tissue, Y-27632, a ROK inhibitor, significantly attenuated the carbachol-induced contraction without changing [Ca 2+]i. Phosphorylation of MLC20 was increased by carbachol and this increased phosphorylation was blocked by treatment of tissue with Y-27632. In tension measurements of single hyperpermeable cells, carbachol evoked sustained contraction at constant pCa 6.7 and these agonist-induced contractions were decreased by treatment with Y-27632. These results suggest that activation of a ROK-mediated signaling pathway(s) plays an important role in agonist-induced alterations in MLC20 phosphorylation and force of rat myometrium.<br>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 65 (1), 43-50, 2003
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681403414400
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- NII Article ID
- 110003886101
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD3sXhs12jt78%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 6444451
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- PubMed
- 12576703
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed