Apoptosis in the Chicken Bursa of Fabricius Induced by X-Irradiation.
Bibliographic Information
- Other Title
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- X線照射によりニワトリヒナのファブリキウス嚢に誘発されるアポトーシス
- Apoptosis in the chicken bursa of fabri
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Description
Immature B lymphocytes in the chicken bursa of Fabricius have previously been reported to undergo apoptosis by low doses of ionizing radiation. In the present study, newly hatched chickens were subjected to whole-body X-irradiation, and the bursa of Fabricius was examined at various postirradiation times by light and electron microscopy to obtain information on the change of ultrastructure of irradiated bursal cells as well as on the time course and dose-response for the induction of apoptosis. Histological examination by light microscopy showed that pyknotic cells started to increase in the bursa within a few hours after irradiation and the frequency of occurrence reached a maximum at 6 hr. An evident increase of the pyknotic cells in number was observed at a dose of as little as 1 Gy, and the frequency increased with increases in the dose, rcaching over 90% at 15 Gy. Electron microscopy of the irradiated bursa revealed typical apoptotic morphology such as chromatin condensation, nuclear fragmentation, formation of apoptotic bodies and phagocytosis of pyknotic cells. Induction of apoptosis was also confirmed by the appearance of a typical DNA ladder pattern on agarose gel. Thus, the present results demonstrate that the chicken bursal cells are hypersensitive to X-irradiation with regard to induction of apoptosis, and that the apoptotic bursal cells exhibit most of the ultrastructural features known to be typical of apoptosis.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 58 (10), 1001-1006, 1996
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681403604096
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- NII Article ID
- 110003916627
- 130003572280
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- NII Book ID
- AA10796138
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- COI
- 1:STN:280:ByiD2szisFc%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 4057955
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- PubMed
- 8916001
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed