Characteristics of Major Structural Protein Coding Gene and Leader-Body Sequence in Subgenomic mRNA of Porcine Reproductive and Respiratory Syndrome Virus Isolated in Japan.
Bibliographic Information
- Other Title
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- 日本で分離された豚繁殖・呼吸障害症候群ウイルスの主要な構造蛋白コード遺伝子及びサブゲノムmRNAの連結遺伝子の解析
- Characteristics of major structural pro
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Abstract
Nucleotide sequence, 1713nt in length, of porcine reproductive and respiratory syndrome virus (PRRSV) isolated in Japan was determined. The sequence encompassed 3 overlapping open reading frames (ORFs), ORF5 to ORF7. These ORFs encodes major structural proteins of PRRSV. The deduced amino acid sequence of each ORF showed higher than 87.5% identity with an American isolate, and lower (54.6 to 80.5%) identity with an European isolate. This result supported a previous report about antigenic characteristics of the EDRD-1 strain. Leader-body junction sequence in subgenomic mRNA of the EDRD-1 strain was determined by sequencing cDNA clones of subgenomic RNAs. A common sequence motif of 5 nucleotide, represented by UA(A/G)CC, was identified as the junction sequence.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 58 (4), 377-380, 1996
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681403743232
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- NII Article ID
- 110003916519
- 130003572379
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DyaK28Xjtlegt74%3D
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- ISSN
- 13477439
- 09167250
- http://id.crossref.org/issn/09167250
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- NDL BIB ID
- 3948449
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- PubMed
- 8741275
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed