Cloning and Chromosomal Assignment of the Porcine Interleukin-2 Receptor Alpha(IL-2R.ALPHA.) Gene.
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- KOKUHO Takehiro
- Department of Biological Product, National Institute of Animal Health
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- HIRAIWA Hideki
- Animal Genome Program Team, STAFF-Institute
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- YASUE Hiroshi
- Animal Genome Research Group, National Institute of Animal Industry
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- WATANABE Satoko
- Department of Biological Product, National Institute of Animal Health
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- YOKOMIZO Yuichi
- Department of Biological Product, National Institute of Animal Health
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- INUMARU Shigeki
- Department of Biological Product, National Institute of Animal Health
Bibliographic Information
- Other Title
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- Cloning and chromosomal assignment of the porcine interleukin-2 receptor alpha (IL-2Rα) gene
- Cloning and chromosomal assignment of the porcine interleukin 2 receptor alpha IL 2R アルファ gene
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Abstract
Porcine genomic DNA encoding a 55 kDa subunit of interleukin-2 receptor (IL-2R), which is termed α chain (IL-2R α), was cloned by repeated plaque hybridization using IL-2Rα cDNA as a probe. Two different lambda phage clones, one of which encoded exon 1 and the 5'-upstream flanking region of IL-2Rα gene and another encoded the sequence from exon 2 to exon 8, were isolated. By analysis of the 5'-upstream region of the gene, putative binding motifs for transcription factors such as GATA family proteins, Ikaros, NF-κB, NF-IL2R α and SRF, were found as described in human, murine and bovine genes. Two additional motifs for STAT4 binding were also found in this region. Moreover, using the FISH technique, we assigned the porcine IL-2Rα locus to the distal end of the long arm of chromosome 10 (10q6-qter) where the vimentin gene had been assigned nearby.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 62 (8), 841-847, 2000
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681404003456
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- NII Article ID
- 110003920445
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD3cXms1yqurw%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 5454545
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- PubMed
- 10993181
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed