Intrauterine embryo transfer with canine embryos cryopreserved by the slow freezing and the Cryotop method

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  • HORI Tatsuya
    Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1–7–1 Kyonan-cho, Musashino-shi, Tokyo 180–8602, Japan
  • USHIJIMA Hitoshi
    Laboratory of Animal Reproduction, Nippon Veterinary and Life Science University, 1–7–1 Kyonan-cho, Musashino-shi, Tokyo 180–8602, Japan
  • KIMURA Taku
    Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1–7–1 Kyonan-cho, Musashino-shi, Tokyo 180–8602, Japan
  • KOBAYASHI Masanori
    Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1–7–1 Kyonan-cho, Musashino-shi, Tokyo 180–8602, Japan
  • KAWAKAMI Eiichi
    Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1–7–1 Kyonan-cho, Musashino-shi, Tokyo 180–8602, Japan
  • TSUTSUI Toshihiko
    Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1–7–1 Kyonan-cho, Musashino-shi, Tokyo 180–8602, Japan International Institute of Small Animal Medicine (Bio Plus), AHB Inc., 3–7–11 Kiba, Koutou-ku, Tokyo 135–0042, Japan

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Canine embryos (8-cell to blastocyst stages) frozen-thawed using the slow-freezing method with glycerol (four recipients) or dimethyl sulfoxide (three recipients) as a cryoprotectant and vitrified-warmed using the Cryotop method (five recipients) were surgically transferred into the unilateral uterine horn of recipient bitches. As a result, the morphology of embryos frozen-thawed using the slow-freezing method was judged to be normal, but no conception occurred in any of the recipient bitches. Two of the five bitches that received transferred embryos (morula to early blastocyst stages) vitrified-warmed using the Cryotop method became pregnant and produced normal pups (1/9 embryos, 11.1% and 1/6 embryos, 17.0%). It was concluded that the Cryotop method was more appropriate for canine embryo cryopreservation than the slow-freezing method, which is used for the cryopreservation of embryos of other mammalian species.

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