Rapid Identification of <i>Mycoplasma pulmonis</i> Isolated from Laboratory Mice and Rats Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
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- GOTO Kazuo
- Department of Clinical Laboratory Medicine, School of Medical Technology, Teikyo University Graduate School of Medical Technology, Teikyo University Department of Clinical Laboratory Medicine, School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan Graduate School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan
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- YAMAMOTO Mikachi
- Laboratory of Space and Environmental Medicine, Graduate School of Medical Technology, Teikyo University Laboratory of Space and Environmental Medicine, Graduate School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan
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- ASAHARA Miwa
- Graduate School of Medical Technology, Teikyo University Graduate School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan
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- TAMURA Takashi
- Graduate School of Medical Technology, Teikyo University Graduate School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan
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- MATSUMURA Mitsuru
- Department of Clinical Laboratory Medicine, School of Medical Technology, Teikyo University Graduate School of Medical Technology, Teikyo University Department of Clinical Laboratory Medicine, School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan Graduate School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan
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- HAYASHIMOTO Nobuhito
- Central Institute for Experimental Animals, ICLAS Monitoring Center Central Institute for Experimental Animals, ICLAS Monitoring Center, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216–0001, Japan
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- MAKIMURA Koichi
- Graduate School of Medical Technology, Teikyo University Laboratory of Space and Environmental Medicine, Graduate School of Medical Technology, Teikyo University Graduate School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan Laboratory of Space and Environmental Medicine, Graduate School of Medical Technology, Teikyo University, 2–11–1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan
Bibliographic Information
- Other Title
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- Rapid Identification of Mycoplasma pulmonis Isolated from Laboratory Mice and Rats Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
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Abstract
Mycoplasma species identification is based on biochemical, immunological, and molecular methods that require several days for accurate identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a novel method for identification of bacteria and has recently been introduced into the clinical microbiology laboratory as a rapid and accurate technique. This method allows a characteristic mass spectral fingerprint to be obtained from whole inactivated mycoplasmal cells. In this study, we evaluated the performance of the MALDI-TOF MS for the identification of Mycoplasma by comparison with standard sequence analysis of 16S rRNA. We developed the first database of MALDI-TOF MS profiles of Mycoplasma species, containing Mycoplasma pulmonis, M. arthritidis, and M. neurolyticum, which are the most common pathogens in mice and/or rats, and species-specific spectra were recorded. Using the database, 6 clinical isolates were identified. Six tracheal swabs from 4 mice and 2 rats were cultured on PPLO agar for 4 to 7 days, and the colonies were directly applied to analyze the protein profiles. Five strains were identified as M. pulmonis, and 1 strain from a mouse was identified as M. neurolyticum (spectral scores were >2.00); the results were consistent with the results of the 16S rRNA gene sequence analysis (homologies>97.0%). These data indicate that MALDI-TOF MS can be used as a clearly rapid, accurate, and cost-effective method for the identification of M. pulmonis isolates, and this system may represent a serious alternative for clinical laboratories to identify Mycoplasma species.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 74 (8), 1083-1086, 2012
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681404464256
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- NII Article ID
- 130001879806
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- NII Book ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC38rks1amsw%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 023958353
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- PubMed
- 22498928
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed