Inhibition of Binding of <i>Vibrio vulnificu</i>s Hemolysin (VVH) by MβCD

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  • SUGIYAMA Hiroyuki
    Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • KASHIMOTO Takashige
    Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • UENO Shunji
    Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan
  • SUSA Nobuyuki
    Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan

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  • Inhibition of Binding of Vibrio vulnificus Hemolysin (VVH) by MβCD

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Abstract

Vibrio vulnificus secrets a pore-forming toxin called Vibrio vulnificus hemolysin (VVH). In this study, we showed that methyl-beta-cyclodextrin (MβCD), an oligosaccharide, decreased binding of VVH to Chinese hamster ovary (CHO) cells, resulting in inhibition of its cytotoxicity. When the VVH was incubated with MβCD, cytotoxicity of the toxin was inhibited from 100.3 ± 7.2% to 19.6 ± 5.3%. Binding analysis showed that the amount of VVH on the cells was decreased from 101.4 ± 9.2% to 18.1 ± 8.0% only when MβCD was present in the culture media. Our results indicate that the inhibition of cytotoxicity of VVH by MβCD was due to a decrease in the amount of toxin binding to CHO cells.

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