Sequence differences in the internal transcribed spacer 1 and 5.8S ribosomal RNA among three <i>Moniezia</i> species isolated from ruminants in Japan
-
- OHTORI Maiko
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3–18–8, Morioka 020–8550, Japan
-
- AOKI Mikiko
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3–18–8, Morioka 020–8550, Japan
-
- ITAGAKI Tadashi
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3–18–8, Morioka 020–8550, Japan
Search this article
Description
This study was designed to clarify the differences in the internal transcribed spacer (ITS) 1 and 5.8S nucleotide sequences of Moniezia expansa, M. benedeni and M. monardi isolated from ruminants in Japan and to determine their phylogenetic relationships. A 98% similarity in the 5.8S sequences was observed among the 3 Moniezia species, whereas many nucleotide indels and substitutions were observed in the ITS1 sequences among the three Moniezia species. These results suggest that the ITS1 region could serve as a potential marker for discriminating the 3 Moniezia species. In the phylogenetic tree based on the ITS1 sequences, M. monardi and M. benedeni showed genetically closer relationship to each other than to M. expansa.
Journal
-
- Journal of Veterinary Medical Science
-
Journal of Veterinary Medical Science 77 (1), 105-107, 2015
JAPANESE SOCIETY OF VETERINARY SCIENCE