First Molecular Characterization of <i>Anaplasma marginale</i> in Cattle and <i>Rhipicephalus </i>(<i>Boophilus</i>)<i> microplus</i> Ticks in Cebu, Philippines
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- YBAÑEZ Adrian Patalinghug
- United Graduate School of Veterinary Sciences, Gifu University, Gifu 501–1193, Japan Department of Veterinary Clinical Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
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- SIVAKUMAR Thillaiampalam
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
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- YBAÑEZ Rochelle Haidee Daclan
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
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- RATILLA Jowarren Catingan
- National Dairy Authority, Cebu City 6000, Philippines
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- PEREZ Zandro Obligado
- GPY Veterinare Animale - Group of Veterinary Clinics, Cebu City 6000, Philippines
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- GABOTERO Shirleny Reyes
- GPY Veterinare Animale - Group of Veterinary Clinics, Cebu City 6000, Philippines
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- HAKIMI Hassan
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan United Graduate School of Veterinary Sciences, Gifu University, Gifu 501–1193, Japan
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- KAWAZU Shin-ichiro
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
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- MATSUMOTO Kotaro
- Department of Veterinary Clinical Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
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- YOKOYAMA Naoaki
- United Graduate School of Veterinary Sciences, Gifu University, Gifu 501–1193, Japan National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
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- INOKUMA Hisashi
- United Graduate School of Veterinary Sciences, Gifu University, Gifu 501–1193, Japan National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
Bibliographic Information
- Other Title
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- First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines
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Abstract
Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect and characterize A. marginale in cattle and ticks in Cebu, Philippines. A total of 12 bovine blood samples and 60 Rhipicephalus (Boophilus) microplus ticks were collected on the Cebu Island in 2011. 16S rRNA-based screening-PCR and DNA sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for A. marginale, and 1 tick (1.7%) to be positive for A. centrale. Selected positive DNA samples were further characterized based on 16S rRNA (longer sequence), Msp5, Msp1α,gltA and groEL genes for phylogenetic analyses. Sequence identities of partial DNA fragments of A. marginale from the Philippines revealed 99.1–100% (16S rRNA, gltA, groEL and Msp5) and 94.3–97.6% (Msp1α) identities to the closest isolates from other countries. Moreover, sequence analysis of the Msp1α, gene showed 3 variants, including a case of co-infection with 2 variants. Phylogenetic analyses based on Msp1α and Msp5 genes revealed that Philippine A. marginale isolates formed a monophyletic lineage, and were phylogenetically related to Brazilian and Chinese isolates. On the other hand, a highly specific and sensitive nested PCR based on groEL, with a detection limit of 2 copies/PCR, was developed to detect A. marginale in the Philippines. This study reported the first molecular detection and characterization of A. marginale in cattle and R. microplus in Cebu, Philippines.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 75 (1), 27-36, 2013
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681405415552
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- NII Article ID
- 130001879686
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- NII Book ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC38fmvVWjug%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 024246024
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- PubMed
- 22878542
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed