Quantitative PCR detection of feline morbillivirus in cat urine samples
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- FURUYA Tetsuya
- Research and Education Center for Prevention of Global Infectious Diseases of Animals, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
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- WACHI Akiko
- Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
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- SASSA Yukiko
- Laboratory of Epizootiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
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- OMATSU Tsutomu
- Research and Education Center for Prevention of Global Infectious Diseases of Animals, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
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- NAGAI Makoto
- Research and Education Center for Prevention of Global Infectious Diseases of Animals, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan Laboratory of Epizootiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
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- FUKUSHIMA Ryuji
- Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
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- SHIBUTANI Makoto
- Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
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- YAMAGUCHI Tomohiro
- Canine-Lab. Inc., 2–24–16 Naka-cho, Venture-Port 301, Koganei-shi, Tokyo 184–0012, Japan
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- UEMATSU Yosuke
- Canine-Lab. Inc., 2–24–16 Naka-cho, Venture-Port 301, Koganei-shi, Tokyo 184–0012, Japan
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- SHIROTA Kinji
- Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252–5201, Japan
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- MIZUTANI Tetsuya
- Research and Education Center for Prevention of Global Infectious Diseases of Animals, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
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抄録
Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 77 (12), 1701-1703, 2015
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681405550464
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- NII論文ID
- 130005115326
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 027052436
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- PubMed
- 26227478
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可