Blood biochemical changes in mice after administration of a mixture of three anesthetic agents

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  • OCHIAI Yuichiro
    Chugai Research Institute for Medical Science, Inc., 1–135 Komakado, Gotemba, Shizuoka 421–8513, Japan Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069–8501, Japan
  • IWANO Hidetomo
    Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069–8501, Japan
  • SAKAMOTO Takako
    Chugai Research Institute for Medical Science, Inc., 1–135 Komakado, Gotemba, Shizuoka 421–8513, Japan
  • HIRABAYASHI Manabu
    Chugai Research Institute for Medical Science, Inc., 1–135 Komakado, Gotemba, Shizuoka 421–8513, Japan
  • KANEKO Eiji
    Chugai Research Institute for Medical Science, Inc., 1–135 Komakado, Gotemba, Shizuoka 421–8513, Japan
  • WATANABE Toshihiko
    Chugai Pharmaceutical Co., Ltd., 1–135 Komakado, Gotemba, Shizuoka 421–8513, Japan
  • YAMASHITA Kazuto
    Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069–8501, Japan
  • YOKOTA Hiroshi
    Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069–8501, Japan

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Currently, from the viewpoint of animal welfare, anesthesia or analgesia is required during experimental procedures in animals that are likely to cause pain. A part of these anesthetics have been reported to influence a blood biochemical level. It is important for us to understand the effect of the anesthetic on blood biochemistry when we choose the anesthetic agent to be used in experiments. In this study, we examined the blood biochemical changes in mice after administration of a new mixture of three anesthetic agents −medetomidine / midazolam / butorphanol (MMB). We subcutaneously administered two dose combinations of MMB (0.45 / 6 / 7.5 and 0.9 / 12 / 15 mg/kg) in mice, followed by administration of atipamezole, for reversal of anesthetic effects, after 1 hr. Thereafter, blood biochemistry was assessed at 1, 4 and 24 hr after MMB administration. We observed that MMB administration caused a transient increase in blood sugar, inorganic phosphorus, potassium and creatine kinase levels. These, however, returned to the reference range 24 hr after MMB administration. In conclusion, MMB changes the levels of some blood biochemical parameters, but not to an extent that would threaten health. However, when using laboratory animals, this effect of MMB may influence the experimental results, depending on the experimental content. Hence, the choice of anesthetic agents used in laboratory animals should be based on detailed knowledge of their pharmacological effects.

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