Key Role of Glycogen Storage in High K<sup>+</sup>-Induced Contraction of the Smooth Muscles of the Bovine Trachea
-
- KANEDA Takeharu
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180–8602, Japan
-
- FUJIEDA Tomoe
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180–8602, Japan
-
- ETO Yuta
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180–8602, Japan
-
- NAGAI Yuta
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180–8602, Japan
-
- SASAKI Noriyasu
- Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Nippon Veterinary and Life Science University Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1–7–1 Kyonan-cho, Musashino, Tokyo 180–8602, Japan
-
- TAJIMA Tsuyoshi
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180–8602, Japan
-
- URAKAWA Norimoto
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180–8602, Japan
-
- SHIMIZU Kazumasa
- Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180–8602, Japan
Bibliographic Information
- Other Title
-
- Pharmacology : Key Role of Glycogen Storage in High K⁺-Induced Contraction of the Smooth Muscles of the Bovine Trachea
Search this article
Abstract
To elucidate the role of glycogen in the contraction of tracheal smooth muscle, we investigated the changes in the glycogen contents of the bovine trachea during contractions induced by high K+ and hypoxia (achieved by bubbling N2 instead of O2), either in a glucose-free condition or in the presence of iodoacetic acid (IAA), an inhibitor of glycolysis. Hyperosmotic addition of 65 mM KCl (H-65 K+) induced a sustained contraction. A glucose-free condition did not affect H-65 K+-induced contraction. However, hypoxia slightly inhibited the contraction, and glucose-free PSS with hypoxia or IAA remarkably inhibited the H-65 K+-induced contraction. H-65 K+ induced a sustained increase in reduced pyridine nucleotide (PNred) fluorescence, representing glycolysis activity. Hypoxia alone slightly enhanced PNred fluorescence, and when combined with a glucose-free condition, it remarkably enhanced the H-65 K+-induced PNred fluorescence. IAA inhibited PNred fluorescence. In the presence of H-65 K+, a glucose-free condition, hypoxia and the combination of glucose-free PSS and hypoxia decreased the glycogen contents. However, IAA had no effect on glycogen contents. Although hypoxia or glucose-free PSS did not affect PCr and ATP contents, the combination of hypoxia and glucose-free PSS or IAA induced a gradual decrease of PCr content. In conclusion, we suggest that endogenous glycogen was utilized to increase the activity of glycolysis for maintaining high K+-induced contraction of the bovine trachea in the glucose -free and/or hypoxic condition.
Journal
-
- Journal of Veterinary Medical Science
-
Journal of Veterinary Medical Science 74 (10), 1277-1282, 2012
JAPANESE SOCIETY OF VETERINARY SCIENCE
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282681405701632
-
- NII Article ID
- 130001879822
-
- NII Book ID
- AA10796138
-
- COI
- 1:STN:280:DC%2BC38npsV2mtQ%3D%3D
-
- ISSN
- 13477439
- 09167250
-
- NDL BIB ID
- 024055433
-
- PubMed
- 22673054
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed