Apoptosis of Canine Mammary Tumor Cells Induced by Small Interfering RNA (siRNA) against Bcl-xL Gene
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- TSUCHIYA Fumito
- Department of Pathobiology, Nihon University School of Veterinary Medicine
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- KANO Rui
- Department of Pathobiology, Nihon University School of Veterinary Medicine
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- SANO Junichi
- Department of Pathobiology, Nihon University School of Veterinary Medicine
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- OGUMA Keisuke
- Department of Pathobiology, Nihon University School of Veterinary Medicine
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- HASEGAWA Atsuhiko
- Department of Pathobiology, Nihon University School of Veterinary Medicine
Bibliographic Information
- Other Title
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- 遺伝子干渉法を用いたイヌのBcl-xLの機能解析
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Description
The inhibition of Bcl-xL mRNA expression and the acceleration of apoptotic cell rates in canine mammary tumor cell line (CF33) by the small interfering RNA (siRNA) were analyzed. The level of Bcl-xL transcripts in CF33 was decreased when cultured with siRNA, suggesting that siRNA might inhibit the expression of Bcl-xL mRNA in the CF33. Apoptotic cell rates in CF33 cultured with siRNA in Oligofectamine medium, with double strand RNA in Oligofectamine medium, without siRNA in Oligofectamine medium and in DMEM alone were 60.9%, 30%, 28.7% and 11.6% at 48-hr incubation, respectively, when evaluated by TUNEL assay. From these results, it was suggested that canine Bcl-xL might be an anticancer target of canine tumors.<br>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 68 (11), 1199-1201, 2006
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681405746560
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- NII Article ID
- 110005664041
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- NII Book ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 8589423
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed