Apoptosis of Canine Mammary Tumor Cells Induced by Small Interfering RNA (siRNA) against Bcl-xL Gene

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  • TSUCHIYA Fumito
    Department of Pathobiology, Nihon University School of Veterinary Medicine
  • KANO Rui
    Department of Pathobiology, Nihon University School of Veterinary Medicine
  • SANO Junichi
    Department of Pathobiology, Nihon University School of Veterinary Medicine
  • OGUMA Keisuke
    Department of Pathobiology, Nihon University School of Veterinary Medicine
  • HASEGAWA Atsuhiko
    Department of Pathobiology, Nihon University School of Veterinary Medicine

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  • 遺伝子干渉法を用いたイヌのBcl-xLの機能解析

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Abstract

The inhibition of Bcl-xL mRNA expression and the acceleration of apoptotic cell rates in canine mammary tumor cell line (CF33) by the small interfering RNA (siRNA) were analyzed. The level of Bcl-xL transcripts in CF33 was decreased when cultured with siRNA, suggesting that siRNA might inhibit the expression of Bcl-xL mRNA in the CF33. Apoptotic cell rates in CF33 cultured with siRNA in Oligofectamine medium, with double strand RNA in Oligofectamine medium, without siRNA in Oligofectamine medium and in DMEM alone were 60.9%, 30%, 28.7% and 11.6% at 48-hr incubation, respectively, when evaluated by TUNEL assay. From these results, it was suggested that canine Bcl-xL might be an anticancer target of canine tumors.<br>

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