Bacteriology : Characterization of Aerococcus viridans isolated from milk samples from cows with mastitis and manure samples

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  • SAISHU Nobukazu
    Tottori Prefectural Kurayoshi Livestock Hygiene Service Center, 2–132 Seidani-cho, Kurayoshi, Tottori 682–0017, Japan The United Graduate School of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
  • MORIMOTO Kazutaka
    Tottori Prefectural Kurayoshi Livestock Hygiene Service Center, 2–132 Seidani-cho, Kurayoshi, Tottori 682–0017, Japan
  • YAMASATO Hiroshi
    Tottori Prefectural Kurayoshi Livestock Hygiene Service Center, 2–132 Seidani-cho, Kurayoshi, Tottori 682–0017, Japan
  • OZAKI Hiroichi
    The United Graduate School of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University, 4–101 Koyama, Tottori 680–8553, Japan
  • MURASE Toshiyuki
    The United Graduate School of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University, 4–101 Koyama, Tottori 680–8553, Japan

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  • Characterization of <i>Aerococcus viridans</i> isolated from milk samples from cows with mastitis and manure samples

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Thirty-eight Aerococcus viridans isolates were obtained from milk from 478 cows with clinical mastitis in a farm during the periods between November 2011 and February 2012, and between December 2012 and March 2013. Additional isolates were obtained from processed manure (a mixture of composted manure, straw and hydrated lime) and bedding materials. The processed manure was later used to cover the floor of the stalls in barns as bedding materials. The temperatures recorded in the composted and processed manure were not as high as those generally observed during satisfactory composting. To reveal the association of A. viridans in manure-related products with intramammary infection in cows, isolates were characterized by their DNA fragment patterns as determined by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Isolates obtained from milk, processed manure and bedding materials had identical DNA fragment patterns. Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials. Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan. In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good’s buffer (pH 9) at high temperature (50°C). The results suggest that the processed manure and bedding materials in this farm were possible sources of A. viridans that caused infection in the cows with mastitis.

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