Deep sequencing of the transcriptome in the anterior pituitary of heifers before and after ovulation
-
- PANDEY Kiran
- Joint Faculty of Veterinary Medicine, Yamaguchi University, Yoshida 1677-1, Yamaguchi-shi, Yamaguchi 753-8515, Japan
-
- MIZUKAMI Yoichi
- Center for Gene Research, Yamaguchi University, Minami Kogushi 1-1-1, Ube-shi, Yamaguchi 755-8505, Japan
-
- WATANABE Kenji
- Center for Gene Research, Yamaguchi University, Minami Kogushi 1-1-1, Ube-shi, Yamaguchi 755-8505, Japan
-
- SAKAGUTI Syuiti
- Institute of Radioisotope Research and Education, Yamaguchi University, Minami Kogushi 1-1-1, Ube-shi, Yamaguchi 755-8505, Japan
-
- KADOKAWA Hiroya
- Joint Faculty of Veterinary Medicine, Yamaguchi University, Yoshida 1677-1, Yamaguchi-shi, Yamaguchi 753-8515, Japan
書誌事項
- 公開日
- 2017
- 資源種別
- journal article
- DOI
-
- 10.1292/jvms.16-0531
- 公開者
- 公益社団法人 日本獣医学会
この論文をさがす
説明
<p>We aimed to determine gene expression patterns in the anterior pituitary (AP) of heifers before and after ovulation via deep sequencing of the transcriptome (RNA-seq) to identify new genes and clarify important pathways. Heifers were slaughtered on the estrus day (pre-ovulation; n=5) or 3 days after ovulation (post-ovulation; n=5) for AP collection. We randomly selected 4 pre-ovulation and 4 post-ovulation APs, and the ribosomal RNA-depleted poly (A)+RNA were prepared to assemble next-generation sequencing libraries. The bovine APs expressed 12,769 annotated genes at pre- or post-ovulation. The sum of the reads per kilobase of exon model per million mapped reads (RPKM) values of all transcriptomes were 599,676 ± 38,913 and 668,209 ± 23,690, and 32.2 ± 2.6% and 44.0 ± 4.4% of these corresponded to the AP hormones in the APs of pre- and post-ovulation heifers, respectively. The bovine AP showed differential expression of 396 genes (P<0.05) in the pre- and post-ovulation APs. The 396 genes included two G-protein-coupled receptor (GPCR) genes (GPR61 and GPR153) and those encoding 13 binding proteins. The AP also expressed 259 receptor and other 364 binding proteins. Moreover, ingenuity pathway analysis for the 396 genes revealed (P=2.4 × 10−3) a canonical pathway linking GPCR to cytoskeleton reorganization, actin polymerization, microtubule growth, and gene expression. Thus, the present study clarified the novel genes found to be differentially expressed before and after ovulation and clarified an important pathway in the AP.</p>
収録刊行物
-
- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
-
Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 79 (6), 1003-1012, 2017
公益社団法人 日本獣医学会