Improved RNA Extraction Method Using the BioMasher and BioMasher Power-Plus

DOI Web Site Web Site PubMed 参考文献4件
  • YAMAMOTO Takuji
    Nippi Research Institute of Biomatrix, 520–11 Kuwabara, Toride, Ibaraki 302–0017, Japan
  • NAKASHIMA Kentaro
    Institute of Neuroscience, Tokushima Bunri University, 1314–1 Sido, Sanuki, Kagawa 769–2101, Japan
  • MARUTA Yukio
    Lab Co., Ltd., 2–12 Kita 27 Nishi 6, Kita-ku, Sapporo, Hokkaido 001–0027, Japan
  • KIRIYAMA Tomomi
    Nippi Research Institute of Biomatrix, 520–11 Kuwabara, Toride, Ibaraki 302–0017, Japan
  • SASAKI Michi
    Nippi Research Institute of Biomatrix, 520–11 Kuwabara, Toride, Ibaraki 302–0017, Japan
  • SUGIYAMA Shunpei
    Lab Co., Ltd., 2–12 Kita 27 Nishi 6, Kita-ku, Sapporo, Hokkaido 001–0027, Japan
  • SUZUKI Kana
    Lab Co., Ltd., 2–12 Kita 27 Nishi 6, Kita-ku, Sapporo, Hokkaido 001–0027, Japan
  • FUJISAKI Hitomi
    Nippi Research Institute of Biomatrix, 520–11 Kuwabara, Toride, Ibaraki 302–0017, Japan
  • SASAKI Jun
    Nippi Research Institute of Biomatrix, 520–11 Kuwabara, Toride, Ibaraki 302–0017, Japan
  • KAKU-USHIKI Yuko
    Nippi Research Institute of Biomatrix, 520–11 Kuwabara, Toride, Ibaraki 302–0017, Japan
  • TANIDA Masatoshi
    Lab Co., Ltd., 2–12 Kita 27 Nishi 6, Kita-ku, Sapporo, Hokkaido 001–0027, Japan
  • IRIE Shinkichi
    Nippi Research Institute of Biomatrix, 520–11 Kuwabara, Toride, Ibaraki 302–0017, Japan
  • HATTORI Shunji
    Nippi Research Institute of Biomatrix, 520–11 Kuwabara, Toride, Ibaraki 302–0017, Japan

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The BioMasher is a disposable homogenizer that was developed to homogenize bovine brain tissue for bovine spongiform encephalopathy diagnosis. Capable of preventing the biohazard risk from infectious samples, it also prevents cross-contamination among samples. The BioMasher is thus widely used in biochemical research, especially for RNA extraction. Here, we tested a novel BioMasher application for RNA extraction from animal and plant tissues. We also developed a grinding machine specific for the BioMasher, named the BioMasher Power-Plus. We developed RNA extraction protocols using the BioMasher combined with the BioMasher Power-Plus. We compared RNA extraction efficiency of the BioMasher with that of the FastPrep and the glass homogenizer. Though the RNA extraction efficiency by the BioMasher was nearly equivalent to that of the FastPrep and the glass homogenizer, sample preparation time was shorter for the BioMasher. The utility of RNA extraction by the BioMasher was examined in mouse, rat, and tomato tissue samples. In the rodent tissues, the highest extraction efficiency of total RNA was from liver, with lowest efficiency from fibrous tissues such as muscle. The quality of extracted total RNA was confirmed by agarose gel electrophoresis which produced highly visible clear bands of 18S and 28S rRNAs. Reproducibility among different operators in RNA extraction from tomato roots was improved by using the BioMasher Power-Plus. The BioMasher and BioMasher Power-Plus provide an effective and easy homogenization method for total RNA extraction from some rodent and plant tissues.

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