[Updated on Apr. 18] Integration of CiNii Articles into CiNii Research

Three-Dimensional Culture of Keratinocytes and the Formation of Basement Membrane for Canine Footpad Substitute

  • YAMAZOE Kazuaki
    Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
  • MIYAMOTO Shuji
    Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
  • HIKOSAKA Yoko
    Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
  • KITAGAWA Koji
    Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
  • WATANABE Kazuhiro
    Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
  • SAKAI Hiroki
    Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
  • KUDO Tadaaki
    Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University

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Other Title
  • 犬の培養肉球の作成

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Abstract

A pad equivalent for a dog was prepared as a substitute for the loss of footpad. In addition to the time course of formation on epidermal morphogenesis, we investigated expressions of α6 integrin subunit as adhesive molecule, and laminin and type IV and VII collagens as extracellular matrices of basement membrane components. Epithelium of the pad equivalent was thick enough to be easily confirmed at 5 days at the air-liquid interface, but many creases appeared on it at 7 days, and it shrank at 10 and 14 days. Keratinocytes were increased in 4 to 5 cell layers at 1 day at the air-liquid interface, differentiating into basal cell layer. Granular and corneal cell layers were confirmed until 5 days, and maintained their shape at least until 14 days. Alpha 6 integrin was expressed at almost the same fluorescent intensity as native pad tissue at 1 day at the dermal-epidermal junction. Laminin and type IV collagen were intermittently expressed at 5 and 10 days, respectively, at the dermal-epidermal junction, and at 14 days the fluorescence showed almost the same intensity as native pad tissue. The expression of type VII collagen was discontinuous at 2 days at the dermal-epidermal junction, but remained as it was at 14 days. The present findings suggested that although the formation of anchoring fibrils in basement membrane was incomplete, the pad equivalent in the dog was reconstructed similar to a native pad by epidermal morphogenesis.<br>

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