Bacteriology: Nitric oxide causes anoikis through attenuation of E-cadherin and activation of caspase-3 in human gastric carcinoma AZ-521 cells infected with Mycoplasma hyorhinis

DOI Web Site Web Site 被引用文献1件 参考文献28件
  • OBARA Hisato
    Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University Will Animal Hospital Department of Applied Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University
  • HARASAWA Ryô
    Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University Department of Applied Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University

書誌事項

タイトル別名
  • Nitric Oxide Causes Anoikis through Attenuation of E-Cadherin and Activation of Caspase-3 in Human Gastric Carcinoma AZ-521 Cells Infected with Mycoplasma hyorhinis

この論文をさがす

抄録

Mycoplasma hyorhinis (M. hyorhinis) infection leads cultured cells to various biological alterations in cell metabolism including apoptosis. Apoptosis induced by M. hyorhinis has mainly been considered to be due to mycoplasmal endonucleases. We previously reported that apoptosis in a human carcinoma cell line AZ-521 infected with M. hyorhinis was enhanced by addition of L-ascorbic acid to cell cultures. Since both L-ascorbic acid addition and M. hyorhinis infection activated cellular iNOS, we examined the hypothesis that nitric oxide (NO) exerts an apoptotic effect on M. hyorhinis-infected cells and down-regulates E-cadherin. In this study, we showed that M. hyorhinis infection activates iNOS mRNA synthesis, NO production, and caspase-3 activity and attenuates E-cadherin mRNA synthesis by quantitative real-time PCR, Griess assay and fluorescence caspase-3 detection. L-NAME decreased the numbers of apoptotic cells through inhibition caspase-3 activity. Our results indicate that NO causes anoikis throughout attenuation of E-cadherin and activation of caspase-3 in human gastric carcinoma cell line AZ-521 cells infected with M. hyorhinis.<br>

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (28)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ