Bacteriology : Evaluation of Recombinant 28 kDa Outer Membrane Protein of Brucella abortus for the Clinical Diagnosis of Bovine Brucellosis in Korea

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  • LIM Jeong Ju
    College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea
  • KIM Dong Hyeok
    College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea
  • LEE Jin Ju
    College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea
  • KIM Dae Geun
    College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea
  • MIN Wongi
    College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea
  • LEE Hu Jang
    College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea
  • RHEE Man Hee
    College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea
  • CHANG Hong Hee
    Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea
  • KIM Suk
    College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea

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  • Evaluation of Recombinant 28 kDa Outer Membrane Protein of <i>Brucella abortus</i> for the Clinical Diagnosis of Bovine Brucellosis in Korea

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Brucella spp. are Gram-negative, facultative, intracellular coccobacilli that are pathogenic to a variety of mammals, including ruminants and humans. The conventional serological test for diagnosing brucellosis in cattle in Korea is the standard tube agglutination test. However, agglutination tests sometimes give false-positive results due to cross-reactions with other pathogens. The outer membrane proteins of Brucella species have been extensively studied for their immunogenicity and serodiagnostic applications. However, an application of B. abortus OMPs for serodiagnosis has not been successfully established. In this study, cloning and expression of B. abortus Omp28, a group 3 antigen, were accomplished by PCR amplification cloning into a pMAL expression system, and purification of a recombinant Omp28 (rOmp28). The immunogenicity of rOmp28 was confirmed by Western blot analysis with Brucella-positive bovine serum. To determine whether rOmp2 has a potential benefit for use in the serodiagnosis of bovine brucellosis, rOmp28-based ELISA and latex bead agglutination test were performed. B. abortus positive (n=122) or negative (n=88) from TAT were positive (118/122, 96.7%) or negative (84/88, 95.4%) in ELISA and were positive (94/122, 77%) or negative (71/88, 81.7%) in that the latex bead agglutination test, respectively. The sensitivity, specificity and accuracy were 96.7, 95.4, 96.2% in ELISA and 77, 80.6, 78.5% in latex bead agglutination test, respectively. These findings suggest that the rOmp28 of B. abortus might be a good candidate for developing serological diagnostic tools for bovine brucellosis.

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