Comparison of Commercial Enzyme-Linked Immunosorbent Assay Kits with Agar Gel Precipitation and Hemagglutination-Inhibition Tests for Detecting Antibodies to Avian Influenza Viruses
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- SHIRAISHI Rikiya
- Research Institute for Animal Science in Biochemistry & Toxicology, 3–7–11 Hashimotodai, Midori-ku, Sagamihara, Kanagawa 252–0132, Japan
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- NISHIGUCHI Akiko
- Animal Quarantine Service of the Ministry of Agriculture, Forestry and Fisheries of Japan, 11–1 Haramachi, Isogo-ku, Yokohama, Kanagawa 235–0008, Japan
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- TSUKAMOTO Kenji
- Research Team for Zoonotic Diseases, National Institute of Animal Health, 3–1–5 Kannondai, Tsukuba, Ibaraki 305–0856, Japan
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- MURAMATSU Masatake
- Research Institute for Animal Science in Biochemistry & Toxicology, 3–7–11 Hashimotodai, Midori-ku, Sagamihara, Kanagawa 252–0132, Japan Present address: NAS Laboratory Co., Ltd., 680 Tokura, Tomisato, Chiba 286–0212, Japan
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Abstract
We evaluated the utility of 5 commercial enzyme-linked immunosorbent assay (ELISA) kits for detecting antibodies to avian influenza viruses. The sensitivities and specificities of the ELISA kits were compared with those of the agar gel precipitation (AGP) and hemagglutination-inhibition (HI) tests. The results suggest that some ELISA kits might not be suitable for monitoring during the early stages of avian influenza virus infections. Therefore, ELISA kits should only be used in conjunction with a profound knowledge about monitoring of avian influenza.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 74 (9), 1217-1220, 2012
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681407275008
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- NII Article ID
- 130001879753
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- NII Book ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC3s%2Fis1Ggsw%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 024012491
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- PubMed
- 23037780
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed