Distribution of Glucagon-Like Peptide (GLP)-2-Immunoreactive Cells in the Chicken Small Intestine: Antigen Retrieval Immunohistochemistry
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- MONIR Mohammad M.
- Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
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- HIRAMATSU Kohzy
- Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu University, Minami-minowa 8304, Kami-ina, Nagano 399–4598, Japan
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- NISHIMURA Kei
- Department of Food Production Science, Graduate School of Agriculture, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
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- TAKEMOTO Chihiro
- Department of Food Production Science, Graduate School of Agriculture, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
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- WATANABE Takafumi
- Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu University, Minami-minowa 8304, Kami-ina, Nagano 399–4598, Japan
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説明
An antigen retrieval method for immunohistochemical staining of glucagon-like peptide (GLP)-2-immunoreactive cells was investigated in the chicken small intestine. GLP-2-immunoreactive cells were observed as open-typed endocrine cells in the villous epithelium and crypts on both antigen retrieval agent-treated and untreated preparations. No obvious differences were detected in morphological features of GLP-2-immunoreactive cells between treated and untreated preparations. The frequencies of occurrence of GLP-2-immunoreactive cells, however, were significantly different in treated and untreated preparations: in the proximal and distal regions of jejunum and ileum obtained from untreated preparations, the frequencies of occurrence were 0.5 ± 0.2, 0.7 ± 0.1, 0.9 ± 0.2 and 1.5 ± 0.3, respectively (cell numbers per mucosal area: cells/mm2, mean ± SD), whereas those from treated sections were 14.7 ± 2.3, 19.8 ± 2.3, 23.5 ± 4.7 and 34.6 ± 4.9 cells/mm2, respectively. These data indicate that this antigen retrieval method is able to make immunoreactive GLP-2 available for detection and that GLP-2 may act as one of the common hormones secreted by L cells in the chicken small intestine.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 76 (4), 565-568, 2014
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681407564416
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- NII論文ID
- 130003382402
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC2c3mt1Gmtw%3D%3D
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- ISSN
- 13477439
- 09167250
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- HANDLE
- 10091/17858
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- NDL書誌ID
- 025510379
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- PubMed
- 24334814
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可