Distribution of Glucagon-Like Peptide (GLP)-2-Immunoreactive Cells in the Chicken Small Intestine: Antigen Retrieval Immunohistochemistry

DOI 機関リポジトリ HANDLE Web Site Web Site ほか1件をすべて表示 一部だけ表示 被引用文献4件 参考文献16件
  • MONIR Mohammad M.
    Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
  • HIRAMATSU Kohzy
    Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu University, Minami-minowa 8304, Kami-ina, Nagano 399–4598, Japan
  • NISHIMURA Kei
    Department of Food Production Science, Graduate School of Agriculture, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
  • TAKEMOTO Chihiro
    Department of Food Production Science, Graduate School of Agriculture, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399–4598, Japan
  • WATANABE Takafumi
    Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu University, Minami-minowa 8304, Kami-ina, Nagano 399–4598, Japan

この論文をさがす

抄録

An antigen retrieval method for immunohistochemical staining of glucagon-like peptide (GLP)-2-immunoreactive cells was investigated in the chicken small intestine. GLP-2-immunoreactive cells were observed as open-typed endocrine cells in the villous epithelium and crypts on both antigen retrieval agent-treated and untreated preparations. No obvious differences were detected in morphological features of GLP-2-immunoreactive cells between treated and untreated preparations. The frequencies of occurrence of GLP-2-immunoreactive cells, however, were significantly different in treated and untreated preparations: in the proximal and distal regions of jejunum and ileum obtained from untreated preparations, the frequencies of occurrence were 0.5 ± 0.2, 0.7 ± 0.1, 0.9 ± 0.2 and 1.5 ± 0.3, respectively (cell numbers per mucosal area: cells/mm2, mean ± SD), whereas those from treated sections were 14.7 ± 2.3, 19.8 ± 2.3, 23.5 ± 4.7 and 34.6 ± 4.9 cells/mm2, respectively. These data indicate that this antigen retrieval method is able to make immunoreactive GLP-2 available for detection and that GLP-2 may act as one of the common hormones secreted by L cells in the chicken small intestine.

収録刊行物

被引用文献 (4)*注記

もっと見る

参考文献 (16)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ