IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa
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- SHIN Sang-Min
- Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561–756, Republic of Korea
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- KIM Suhee
- Department of Biochemistry, School of Dentistry, Chonnam National University, Gwangju 500–757, Republic of Korea
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- HONG Jin-Gi
- Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561–756, Republic of Korea
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- KIM Yong-Jun
- Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561–756, Republic of Korea
書誌事項
- 公開日
- 2014
- 資源種別
- journal article
- DOI
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- 10.1292/jvms.14-0049
- 公開者
- 公益社団法人 日本獣医学会
この論文をさがす
説明
The aim of study was to evaluate the effects of insulin-like growth factor I (IGF-I) on canine sperm function during cooled and freeze-thaw storage. Extenders supplemented with different IGF-I concentrations (0, 100 and 200 ng/ml) were added to canine spermatozoa, and the sperm samples were stored at 4°C for 48 hr or freeze-thawed. Sperm motility, morphology, plasma-membrane integrity (PMI) and mitochondrial membrane potential (MMP) were evaluated. IGF-I had no effect on PMI or morphology during cooling and freeze-thawing. However, IGF-I alleviated the reduction in progressive motility and MMP caused by cooled storage and led to an improvement in MMP after freeze-thawing. In conclusion, IGF-I can be helpful to maintain progressive motility of canine spermatozoa during hypothermic storage via increased MMP.
収録刊行物
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- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
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Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 76 (7), 1065-1067, 2014
公益社団法人 日本獣医学会
