Molecular Analysis of Cryptosporidium parvum HNJ-1 Isolated in Japan

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  • AMER Said
    Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University Department of Zoology, Faculty of Science, Kafr El Sheikh University
  • MATSUBARA Ryuma
    Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University
  • MURAKOSHI Fumi
    Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University
  • NAKAI Yutaka
    Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University

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  • Parasitology: Molecular analysis of Cryptosporidium parvum HNJ-1 isolated in Japan

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Abstract

Cryptosporidium parvum HNJ-1 is widely used as a reference strain in Japan. In the present study, the parasite was subjected for further molecular analysis including transcribed ribosomal region (ITS rRNA), dihydrofolate reductase (DHFR) and surface glycoprotein (GP60) genes. Partial sequence analysis of these genes indicated extensive polymorphism in ITS region compared with relevant sequences of other Cryptosporidium parvum isolates. In addition, this strain was identified as C. parvum IIaA15G2R1 subtype, based on the sequence results of GP60 gene locus.<br>

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