Clinical pathology: Effect of NEFA and glucose levels on CPT-I mRNA expression and translation in cultured bovine hepatocytes

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  • XU Chuang
    Department of Clinical Veterinary Medicine, Animal Science and Technology College, Heilongjiang Bayi Agricultural University
  • WANG Zhe
    Department of Clinical Veterinary Medicine, Animal and Veterinary College, Jilin University
  • ZHANG Ri-he
    Department of Clinical Veterinary Medicine, Animal and Veterinary College, Jilin University
  • ZHANG Hong-you
    Department of Clinical Veterinary Medicine, Animal Science and Technology College, Heilongjiang Bayi Agricultural University
  • FU Shi-xin
    Department of Clinical Veterinary Medicine, Animal Science and Technology College, Heilongjiang Bayi Agricultural University
  • XIA Cheng
    Department of Clinical Veterinary Medicine, Animal Science and Technology College, Heilongjiang Bayi Agricultural University

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  • Effect of NEFA and Glucose Levels on CPT-I mRNA Expression and Translation in Cultured Bovine Hepatocytes

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The objective of this study was to determine the effects of NEFA and glucose on carnitine palmitoyltransferase-I (CPT-I) mRNA expression in cultured bovine hepatocytes using real-time reverse transcription polymerase chain reaction and ELISA methods. The results indicated that CPT-I transcription increased gradually, but that CPT-I translation was not significantly changed, with glucose concentrations ranging from 0 to 3.0 mmol/L (P<0.01). Furthermore CPT-I transcription and translation were enhanced significantly when the NEFA concentrations increased from 0 to 1.2 mmol/L and decreased significantly when the NEFA concentrations increased from 1.2 to 4.8 mmol/L (P<0.01). A high concentration NEFA was found to reduce fatty acid oxidation, potentially explaining the development from NEB to ketosis in dairy cows.<br>

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