A Real-Time PCR for Detection and Quantification of <i>Mycoplasma ovipneumoniae</i>

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  • YANG Falong
    Department of Veterinary Medicine, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • DAO Xiaofang
    Department of Veterinary Medicine, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • RODRIGUEZ-PALACIOS Alex
    Division of Gastroenterology and Liver Disease, Case Western Reserve University, Cleveland, OH 44106, U.S.A.
  • FENG Xufei
    Department of Veterinary Medicine, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • TANG Cheng
    Department of Veterinary Medicine, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • YANG Xiaonong
    Department of Veterinary Medicine, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • YUE Hua
    Department of Veterinary Medicine, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China

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Other Title
  • Bacteriology : A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae
  • A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae

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Abstract

A real-time PCR for detection and quantification of M. ovipneumoniae was developed using 9 recently sequenced M. ovipneumoniae genomes and primers targeting a putative adhesin gene p113. The assay proved to be specific and sensitive (with a detection limit of 22 genomic DNA) and could quantify M. ovipneumoniae DNA over a wide linear range, from 2.2 × 102 to 2.2 × 107 genomes.

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