Development and evaluation of the internal-controlled real-time PCR assay for Rhodococcus equi detection in various clinical specimens
-
- STEFAŃSKA Ilona
- Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 8 Ciszewskiego St., 02–786 Warsaw, Poland Department of Fermentation Technology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, 36 Rakowiecka St., 02–532 Warsaw, Poland
-
- WITKOWSKI Lucjan
- Laboratory of Veterinary Epidemiology and Economics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 159c Nowoursynowska St., 02–776 Warsaw, Poland
-
- RZEWUSKA Magdalena
- Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 8 Ciszewskiego St., 02–786 Warsaw, Poland
-
- DZIECIĄTKOWSKI Tomasz
- 4)Chair and Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinski St., 02–004 Warsaw, Poland
書誌事項
- タイトル別名
-
- Development and evaluation of the internal-controlled real-time PCR assay for <i>Rhodococcus equi</i> detection in various clinical specimens
この論文をさがす
説明
Rhodococcus equi is the causative agent of rhodococcosis in horses, resulting in significant morbidity and mortality in foals. This bacterium has also been isolated from a variety of animals and is being increasingly reported as a cause of infection in humans, mainly in immunosuppressed individuals. Laboratory diagnostics of R. equi infections based only on conventional microbiological methods shows low accuracy and can lead to misidentification. The objective of the study was to develop and evaluate a real-time PCR assay for direct detection of R. equi in various clinical specimens, including tissue samples. The species-specific region of the gene encoding R. equi cholesterol oxidase, choE, was used as a qPCR-target. The diagnostic applicability of the assay was confirmed by testing various tissue specimens obtained from horses with clinical signs of rhodoccocal infection and swine submaxillary lymph nodes. The rate of R. equi detection in clinical specimens by the developed assay was higher in comparison to the culture method (90% vs. 60.0% of positive samples) and conventional PCR (90.0% vs. 20.0% of positive samples). In case of 13 samples that were negative in the culture-based method, R. equi was detected by the developed assay. Only in one case, it gave negative result for culture-positive sample. The assay may provide a simple and rapid tool to complement the classical methods of R. equi detection based on culture and phenotypic identification of isolates, as the performed evaluation indicated a high specificity and accuracy of the results.
収録刊行物
-
- The Journal of Veterinary Medical Science
-
The Journal of Veterinary Medical Science 78 (4), 543-549, 2016
公益社団法人 日本獣医学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681407890560
-
- NII論文ID
- 130007331488
-
- NII書誌ID
- AA10796138
-
- ISSN
- 13477439
- 09167250
-
- NDL書誌ID
- 027341315
-
- PubMed
- 26655770
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可