Studies on Acid Polysaccharides in Blood Vessel Wall (I) : STUDIES ON ACID POLYSACCHARIDES IN BLOOD VESSEL WALL WITH HISTO-CHEMICAL TECHNIQUE

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The author has studied the acid polysaccharides of arterial and venous walls with histo-chemical technique applying many dyeing methods and fermentative procedures, and has also investigated the possibility of detecting acid polysaccharides in capillary walls.By using the three staining methods (P.A.S. stain, colloid stain and tobuidine blue metachromasia stain) the author recognized that blood vessel walls contain acid polysaccharides from the intima through the media. After digestion by hyaluronidase or chondromucinase the red M. or the bright blue color of artery or vein disappeared, and the same results were obtained using elastase and trypsin. The former is probably explained by depolymerization of acid polysaccharides, and the latter is considered that protein combined with acid polysaccharides was decomposed, and the polysaccharides were liberated and dissolved away. Moreover, arterial walls exhibited M. at pH 1.0 toluidine blue or with toluidine blue in 1/1, 280, 000 solution, and also exhibited basophilia. But, vein remained all negative. That is, the M. substance in arterial walls is "cho" sulfate. The M. substance in venous walls is digested by chondromucinase ; this fact shows that the M. substance in venous walls is also "cho" sulfate, but considering the results of other staining methods it may be polysaccharides of a weak acid, namely "hi" acid. Further investigation is needed. Although the author tried many methods to study cement substance in capillary blood vessels, he could not prove by histo-chemical methods that it includes acid polysaccharides.

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