鏡検によるカンジダの定量的評価についての検討

  • 岡本 真理子
    鶴見大学歯学部附属病院口腔機能診療科
  • 鴨井 美帆
    鶴見大学歯学部附属病院口腔機能診療科
  • 鶴本 明久
    鶴見大学歯学部地域歯科保健学教室
  • 山近 重生
    鶴見大学歯学部附属病院口腔機能診療科
  • 今村 武浩
    鶴見大学歯学部附属病院口腔機能診療科
  • 山本 健
    鶴見大学歯学部附属病院口腔機能診療科 鶴見大学歯学部高齢者歯科学講座
  • 門松 伸一
    鶴見大学歯学部附属病院口腔機能診療科 鶴見大学歯学部高齢者歯科学講座
  • 西岡 千賀子
    鶴見大学歯学部附属病院口腔機能診療科
  • 斎藤 一郎
    鶴見大学歯学部附属病院口腔機能診療科 鶴見大学歯学部病理学講座
  • 前田 伸子
    鶴見大学歯学部口腔微生物学講座
  • 中川 洋一
    鶴見大学歯学部附属病院口腔機能診療科

書誌事項

タイトル別名
  • Quantitative evaluation of <i>Candida</i> by microscopy
  • キョウケン ニ ヨル カンジダ ノ テイリョウテキ ヒョウカ ニ ツイテ ノ ケントウ

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抄録

The purpose of this study was to investigate the quantitative relationship between Candida detected in microscopic and culture examinations.<br>A total of 82 samples obtained from patients at the Dry Mouth Clinic, Tsurumi University Dental Hospital were enrolled in this study. The patients were either clinically diagnosed to have erythematous candidiasis (EC) or suspected to have Candida glossitis (CG) and underwent both microscopy of a smear specimen stained with fluorescent dye, Fungiflora Y, and fungal culture examination. The specimens were obtained from the surface of the dorsum of the tongue by swabbing. The number of Candida was expressed as the number of fungi/field of vision(FOV)by microscopy, and colony-forming units (CFU) in the culture.<br>The results of microscopic and culture examination demonstrated that there were 0 to 3.7 fungi/FVO and 0 to 1992 CFU/dish, respectively. The correlation coefficient of the examinations was 0.569 (p < 0.001) in the EG group and 0.645 (p < 0.001) in the CG group; the regression equation was [CFU/dish] = 330.4 × [number of fungi/FOV] + 124.6, and [CFU/dish] = 304.2 × [number of fungi/FOV] + 54.5, respectively.<br>The study suggested that the direct examination of a smear was useful, even for quantitative evaluations, because a positive correlation was observed between the number of Candida detected by microscopic and culture examinations.

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