Studies on the Steroid Binding Protein

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  • MATSUMOTO Seiji
    Department of Gynecology and Clinical Research, Research Institute of Microbial Diseases, Osaka University

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Other Title
  • 血清のsteroids結合蛋白分画に関する実験的研究
  • 血清のSteroids結合蛋白分画に関する実験的研究-1-"Transcortin"の同定について
  • ケッセイ ノ Steroids ケツゴウ タンパク ブンカク ニ カンスル ジッケンテキ ケンキュウ 1 Transcortin ノ ドウテイ ニ ツイテ
  • I. Determination of “Transcortin”
  • 第1報 “Transcortin” の同定について

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Abstract

Steroid binding protein in human serum was analyzed with the technique of gel filtration and radio-immunoelectrophoresis.<BR>“Transcortin” which we have named as high affinity for the binding of corticosteroid, may be associated with α1-glycoprotein.<BR>Serum of twety normal adults and five patients of uterine cancer was analyzed for steroid binding protein. After incubation of C14-steroid with serum in vitro at 4°C for 40 min., protein fraction of each serum was collected by gel filtration, then concentrated by dialysis using cellulose tube in vacuo. Aliquot of sample was carried out immunoelectrophoresis ; horse anti-human serum (prepared by our Research Institute) was used.<BR>Radioautograms in normal serum revealed the 5 distinct traces of radioactivity associated with the arcs ofρ1 albumin, α1-, α2-lipoprotein, α1-glycoprotein, but only 3 traces (α1-, α2-lipoprotein, and albumin) in cases of hypoproteinemia were observed.<BR>And these results were obtained in each experiment of cortisol, progesterone and estradiol.<BR>From these observations, it is considered that “Transcortin” does not mean only a single protein fraction but involves α1-, α2-lipoprotein and α1-glycoprotein.

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