書誌事項
- タイトル別名
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- A New Method for the Determination of Serum Human Placental Lactogen (H P L) by Single Radial Immunodiffusion
- イチゲン メンエキ カクサンバンホウ ニヨル アタラシイ ケッセイ HPL ソ
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説明
The general radioimmunoassay (RIA) currently employed for the determination of serum HPL requires a special apparatus and a complicated technique. Therefore, in order to simplify the assay we tried to develop a new method using single radial immunodiffusion (SRID).<BR>Basic examinations were made to establish a simplified HPL assay method by SRID. The optimal conditions finally decided for SRID were as follows : the thickness of the agar plate, 1.5mm; the diameter of the well, 3mm; the amount of antigen added, 10μl; antibody concentration, 1.0% for the measurement range from 1.0μg/ml to 16μg/ml and 0.5% for the measurement range from 0.25μg/ml to 1.0pg/ml.<BR>Under these conditions serum HPL was detectable after the 9th week of gestation, and antigen was measurable over 0.25μg/ml. Serum HPL values measured by RIA and SRID from the 9th to the 42nd week of gestation in 138 normal pregnant women were identical, and the correlation was highly significant (r=0.842, p<0.001) <BR>This SRID method was as reliable and sensitive as the RIA and made it easier than the RIA to measure the serum HPL of pregnant women. Therefore, with regard to placental function its clinical application should prove to be certainly beneficial.
収録刊行物
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- Folia Endocrinologica Japonica
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Folia Endocrinologica Japonica 54 (11), 1281-1289, 1978
The Japan Endocrine Society
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詳細情報 詳細情報について
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- CRID
- 1390282681415987200
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- NII論文ID
- 130001938338
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- NII書誌ID
- AN00195891
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- COI
- 1:CAS:528:DyaE1MXotlansA%3D%3D
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- NDL書誌ID
- 1971857
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- ISSN
- 00290661
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- PubMed
- 102542
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可