カイコの中腸皮膜組織のfreeze-etching法による電子顕微鏡観察

DOI 日本農学文献記事索引 Web Site 被引用文献1件

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タイトル別名
  • The Ultrastructure of the Larval Midgut of <i>Bombyx mori</i> in Frozen Sections
  • カイコ ノ チュウ チョウ ヒマク ソシキ ノ freeze-etchlngホウ ニ ヨル デンシ ケンビキョウ カンサツ

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説明

In ultrastructural observations on the midgut of Bombyx larvae utilizing conventional ultrathin sections, there were problems of interpretation with respect to the three dimensional ultrastructure of the nuclear pores, the mitochondria-filled cytoplasmic projections of the goblet cells, and the secretory globules from the columnar cells. This paper reports the results of studies on these problems using an improved frozen section method. The shapes, sizes, and distribution of the nuclear pores were determined. The three dimensional ultrastructure of the mitochondria-filled cytoplasmic projections of the goblet cell was clarified. The three dimensional morphologies of organelles such as the Golgi apparatus, endoplasmic reticulum, microvilli, secretory globules, cell junctions, and basal infoldings in both columnar and goblet cells are described. The utilization of the frozen section method for the electron microscopic study of insect tissues is discussed.

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